Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: Correlation between TRIM27 expression and clinicopathologic features in patients with renal cancer

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Expressing

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: TRIM27 is upregulated and correlated with poor prognosis in human renal cancer. A . The mRNA level of TRIM27 was upregulated in renal cancer. Data were collected from the TCGA database. *** p < 0.001 vs pare-non-cancer tissues B . Prognosis of RCC (KIRC) patients with high or low expression of TRIM27 derived from the TCGA database. C . The relative mRNA level of TRIM27 was upregulated in human renal cancer tissues. n = 20, *** p < 0.001 vs pare-non-cancer tissues. D – F . KEGG, REACTOME, and DUTTA analysis indicated that cell apoptosis was positively correlated with elevated TRIM27 expression in RCC from the database GSE53757. NES: Normalized enrichment score

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Expressing, Derivative Assay

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: TRIM27 silencing suppressed the proliferation and promoted apoptosis of human renal cells. A & B . TRIM27 siRNAs deeply suppressed the relative mRNA and protein content of TRIM27 in human Caki-2 and 786–0 cells, respectively. *** p < 0.001 vs siNC. The mRNA levels of TRIM27 was used 2 −ΔΔCt method and normalized to that of GAPDH. C & D . siTRIM27–1 and siTRIM27–2 significantly suppressed the proliferation of Caki-2 and 786–0 cells. * p < 0.05 vs siNC, *** p < 0.001 vs siNC. E . Apoptosis of Caki-2 and 786–0 cells was significantly increased following transfecting with siTRIM27–1 and siTRIM27–2 at 48 h, respectively. *** p < 0.001 vs siNC. F & G . Western blot was used to examine the protein contents of TRIM27, cleaved caspase-3, NF-κB (cytoplasm), NF-κB (nuclear) and IkBa in Caki-2 cells ( F ) and 786–0 cells ( G ) transfected with siNC, siTRIM27–1, or siTRIM27–2 at 48 h respectively. * p < 0.05 vs siNC, ** p < 0.01 vs siNC, *** p < 0.001 vs siNC

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Western Blot, Transfection

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: oeTRIM27 rescues the function of TRIM27 in siTRIM27-transfected cells. A and B . The proliferations of Caki-2 and 786–0 cells transfected with siTRIM27, oeTRIM27, or siTRIM27 + oeTRIM27 at 0, 12, 24, and 48 h respectively. * p < 0.05 vs Control, *** p < 0.001 vs Control. C and D . Flow cytometric evaluation of the apoptosis of Caki-2 and 786–0 cells transfected with siTRIM27, oeTRIM27, or siTRIM27 + oeTRIM27 at 48 h respectively, *** p < 0.001 vs Control. D and E . Western blot was used to examine the relative proteins of TRIM27, cleaved caspase-3, NF-κB (cytoplasm), and NF-κB (nuclear) in Caki-2 and 786–0 cells transfected with siTRIM27, oeTRIM27, or siTRIM27 + oeTRIM27 at 48 h respectively. *** p < 0.001 vs Control

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Transfection, Control, Western Blot

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: TRIM27 silencing reduced the tumorigenicity of human Caki-2 cells in vivo. A & B . Knockdown of TRIM27 in Caki-2 cells deeply suppressed the tumor volume and weight in vivo. ** p < 0.01 vs siNC, *** p < 0.001 vs siTRIM27. C . TUNEL staining assays were used to determine tumor cell apoptosis. D . TUNEL quantification of siNC and siTRIM27 tumors, *** p < 0.001 vs siNC

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: In Vivo, Knockdown, TUNEL Assay, Staining

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: TRIM27 overexpression promoted the tumorigenicity of human Caki-1 cells in vivo. A & B . Overexpression of TRIM27 in Caki-2 cells significantly increased the tumor volume and weight in vivo. * p < 0.05 vs oeNC, *** p < 0.001 vs oeNC. C . TUNEL staining assays were used to determine tumor cell apoptosis. D . TUNEL quantification of oeNC and oeTRIM27 tumors, *** p < 0.001 vs oeNC

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Over Expression, In Vivo, TUNEL Assay, Staining

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: The NF-κB inhibitor PDTC suppressed the function of oeTRIM27 in Caki-1 cells. A & B . oeTRIM27 significantly upregulated the relative mRNA and protein levels of TRIM27 in Caki-1 cells. *** p < 0.001 vs oeNC. The mRNA levels of TRIM27 was used 2 −ΔΔCt method and normalized to that of GAPDH. C . The proliferation of oeTRIM27 transfected cells was deeply downregulated in the presence of the inhibitor PDTC. * p < 0.05 vs oeNC + DMSO, *** p < 0.001 vs oeNC + DMSO;! p < 0.05 vs oeTRIM27 + DMSO,!! p < 0.01 vs oeTRIM27 + DMSO,!!! p < 0.001 vs oeTRIM27 + DMSO. D . The inhibitor PDTC significantly reduced the apoptosis of oeTRIM27 transfected cells. * p < 0.05 vs oeNC + DMSO, *** p < 0.001 vs oeNC + DMSO;!!! p < 0.001 vs oeTRIM27 + DMSO. E . The nuclear translocation of NF-κB and IkBa in oeNC or oeTRIM27 transfected cells were deeply suppressed by the inhibitor PDTC. *** p < 0.001 vs oeNC + DMSO;!!! p < 0.001 vs oeTRIM27 + DMSO. F . Correlation analysis between TRIM27 and NF-κB in human renal cancer tissues ( n = 20)

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Transfection, Translocation Assay

Journal: BMC Cancer

Article Title: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

doi: 10.1186/s12885-021-08562-5

Figure Lengend Snippet: TRIM27 interacted with Iκbα and was positively associated with its ubiquitination in human Caki-2 cells. A . Western blot was used to examine the protein content of TRIM27 and Iκbα in oeNC or oeTRIM27 with or without the MG132 treatment. *** p < 0.001 vs oeNC + DMSO;!!! p < 0.001 vs oeTRIM27 + DMSO. B . TRIM27 interacted with Iκbα in human Caki-2 cells. C . TRIM27 silencing suppressed the ubiquitination of Iκbα in human Caki-2 cells

Article Snippet: The primary antibodies that used in this study were listed as follows: TRIM27 (#15099; CST, USA), NF-kB (#8242; CST, USA), cleaved caspase 3 (Ab32042; Abcam, UK), H3 (#4499; CST, USA), GAPDH (#5174; CST, USA).

Techniques: Ubiquitin Proteomics, Western Blot

Journal: eLife

Article Title: The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer

doi: 10.7554/eLife.57679

Figure Lengend Snippet:

Article Snippet: Antibody , anti-TRIM27 (Rabbit monoclonal) , Cell Signaling Technology , Cat #15099; RRID: AB_2798707 , IB (1:1000).

Techniques: Recombinant, In Situ, Software